Hematopoietic Stem/Progenitor Cell Enumeration in Apheresis Collections Using a New Microfluidic Cartridge-Based Flow Cytometry Platform
Successful hematopoietic reconstitution after stem cell transplantation depends on the dose of CD34+ stem/progenitor cells present in the transplanted grafts. CD34+ cell phenotyping and enumeration are critical quality control (QC) assays performed during graft processing and characterization. Our Cellular Therapy Laboratory (CTL) relies on the institutional flow cytometry laboratory for such assays. While highly reliable, the current workflow and turnaround time affect clinical practice through additional, potentially unnecessary procedures and CD34+ cell yields above collection targets, altogether causing increased resource and workforce utilization. Hence, we initiated a process development and validation study to test feasibility and performance of a new CD34+ cell phenotyping and enumeration method that has the potential to improve the efficiency of product QC within the CTL.
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